CGSpaceA Repository of Agricultural Research Outputs
    View Item 
    •   CGSpace Home
    • International Institute of Tropical Agriculture (IITA)
    • IITA Journal Articles
    • View Item
       
    • CGSpace Home
    • International Institute of Tropical Agriculture (IITA)
    • IITA Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds

    Thumbnail
    View/Open
    Journal Article (1.521Mb)
    Authors
    Ita, E.E.
    Uyoh, E.A.
    Nakamura, I.
    Ntui, V.O.
    Date
    2020-05
    Language
    en
    Type
    Journal Article
    Review status
    Peer Review
    ISI journal
    Accessibility
    Open Access
    Usage rights
    CC-BY-4.0
    Metadata
    Show full item record
    Share
    
    Citation
    Ita, E.E., Uyoh, E.A., Nakamura, I. & Ntui, V.O. (2020). Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds. South African Journal of Botany, 130, 123-129.
    Permanent link to cite or share this item: https://hdl.handle.net/10568/108838
    DOI: https://doi.org/10.1016/j.sajb.2019.12.022
    Abstract/Description
    Dioscorea rotundata is an economically important food crop in many tropical countries as many people in this region depend on it for food and livelihood. Viral diseases, especially Yam mosaic virus (YMV), constitute a major constraint in the cultivation of this crop as they perpetuate through generations in the vegetatively propagated planting materials. Getting resistant or at least virus-free planting materials for farmers thus becomes crucial. This study was aimed at eliminating YMV in Dioscorea rotundata by cryotherapy of axillary buds. Enlarged axillary buds of YMV-infected TDr 2269 were frozen in liquid nitrogen for 1 h, re-warmed at 40 °C and cultured to regenerate plantlets. Approximately 76.33% plantlet regeneration and 100% YMV eradication were obtained for cryo-treated buds, against 95% and 0% obtained respectively for non-treated buds. RT-PCR and RT-qPCR analyses did not reveal detectable quantity of YMV in treated plants but did in control plants. Plants from cryo-treated buds showed no mosaic symptoms and produced slightly more tubers, and heavier mini-tubers (20.48±3.11 g) under greenhouse conditions contrary to non-treated plants that showed severe mosaic symptoms with significantly smaller tubers (1.91±0.39 g) (P < 0.05). This is the first report showing the elimination of YMV from infected white yam stock plant by cryotherapy and would be useful for producing clean planting materials.
    CGIAR Author ORCID iDs
    Ntuihttps://orcid.org/0000-0003-4709-8087
    Notes
    Open Access Journal
    CGIAR Affiliations
    Roots, Tubers and Bananas
    AGROVOC Keywords
    dioscorea rotundata; yams; plant viruses; breeding methods; seedlings; white yam; mosaic viruses; buds
    Subjects
    AGRONOMY; PLANT BREEDING; PLANT DISEASES; PLANT HEALTH; PLANT PRODUCTION; YAM
    Countries
    Germany
    Regions
    ACP; Europe
    Investors/sponsors
    Bill & Melinda Gates Foundation
    Collections
    • IITA Journal Articles [4608]
    • RTB Journal Articles [1299]

    AboutPrivacy StatementSend Feedback
     

    My Account

    LoginRegister

    Browse

    All of CGSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CCAFS subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy Bioversity subjectBy CIAT subjectBy CIP subjectBy CGIAR System subjectBy Alliance Bioversity–CIAT subjectThis CollectionBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CCAFS subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy Bioversity subjectBy CIAT subjectBy CIP subjectBy CGIAR System subjectBy Alliance Bioversity–CIAT subject

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    AboutPrivacy StatementSend Feedback