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dc.contributor.authorKanduma, E.G.
dc.contributor.authorMwacharo, Joram M.
dc.contributor.authorSunter, J.D.
dc.contributor.authorNzuk, I.
dc.contributor.authorMwaura, S.
dc.contributor.authorKinyanjui, P.W.
dc.contributor.authorKibe, M.
dc.contributor.authorHeyne, H.
dc.contributor.authorHanotte, Olivier H.
dc.contributor.authorSkilton, Robert A.
dc.contributor.authorBishop, Richard P.
dc.date.accessioned2012-07-19T14:16:30Z
dc.date.available2012-07-19T14:16:30Z
dc.date.issued2012-06-30
dc.identifier.citationKanduma, E.G., Mwacharo, J.M., Sunter, J.D., Nzuki, I., Mwaura, S., Kinyanjui, P.W., Kibe, M., Heyne, H., Hanotte, O., Skilton, R.A. and Bishop, R.P. 2012. Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus. Ticks and Tick-borne Diseases 3(3):128-136.en_US
dc.identifier.urihttp://hdl.handle.net/10568/21214
dc.description.abstractBiological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick vector remain unknown due to the absence of appropriate genetic markers. Here, we describe the development and evaluation of a panel of EST micro- and minisatellite markers to characterize the genetic diversity within and between populations of R. appendiculatus and other rhipicephaline species. Sixty-six micro- and minisatellite markers were identified through analysis of the R. appendiculatus Gene Index (RaGI) EST database and selected bacterial artificial chromosome (BAC) sequences. These were used to genotype 979 individual ticks from 10 field populations, 10 laboratory-bred stocks, and 5 additional Rhipicephalus species. Twenty-nine markers were polymorphic and therefore informative for genetic studies while 6 were monomorphic. Primers designed from the remaining 31 loci did not reliably generate amplicons. The 29 polymorphic markers discriminated populations of R. appendiculatus and also 4 other Rhipicephalus species, but not R. zambeziensis. The percentage Principal Component Analysis (PCA) implemented using Multiple Co-inertia Analysis (MCoA) clustered populations of R. appendiculatus into 2 groups. Individual markers however differed in their ability to generate the reference typology using the MCoA approach. This indicates that different panels of markers may be required for different applications. The 29 informative polymorphic micro- and minisatellite markers are the first available tools for the analysis of the phylogeography and population genetics of R. appendiculatus.en_US
dc.language.isoenen_US
dc.sourceTicks and Tick-borne Diseasesen_US
dc.subjectTICKSen_US
dc.subjectPARASITESen_US
dc.titleMicro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatusen_US
dc.typeJournal Articleen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriANIMAL HEALTHen_US
cg.subject.ilriEPIDEMIOLOGYen_US
cg.subject.ilriLIVESTOCKen_US
cg.identifier.statusLimited Accessen_US
cg.identifier.doihttps://dx.doi.org/10.1016/j.ttbdis.2012.05.001en_US
cg.contributor.crpLivestock and Fish


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