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    Generation of monoclonal antibodies to the anti-trypanosomal drug isometamidium

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    Authors
    Peregrine, A.S.
    Eisler, M.C.
    Katende, J.
    Flynn, J.N.
    Gault, E.A.
    Kinabo, L.D.B.
    Holmes, P.H.
    Date Issued
    1994
    Language
    en
    Type
    Journal Article
    Accessibility
    Limited Access
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    Citation
    Hybridoma;13(4): 289-294
    Permanent link to cite or share this item: https://hdl.handle.net/10568/28676
    Abstract/Description
    Mice were immunized with either an isometamidium-human serum albumin (HSA) conjugate or an isometamidium-porcine thyroglobulin conjugate (PTG). Thereafter, monoclonal antibodies (MAbs) IL-A 1001, IL-A 1002, IL-A 1003, 5F7.B7, and 5F7.C9 were generated and selected on the basis that they recognized conjugated and unconjugated isometamidium, but lacked cross-reactivity with the carrier molecules. All five MAbs were of the IgG<sub>1 isotype. Each of the five MAbs was assessed in a competitive ELISA for isometamidium; in each case, the minimum level of detection was approximately 10ng/ml. Each MAb exhibited approximately 0.1% cross-reactivity with the anti-trypanosomal compound diminazene. However, based on their cross-reactivity with the anti-trypanosomal compound homidium, the MAbs could be divided into two groups; IL-A 1001, IL-A 1002, and IL-A 1003, produced using an isometamidium-HSA conjugate as an immunogen, exhibited low levels of cross-reactivity (approximately 0.1%). In contrast, 5F7.B7 and 5F7.C9, produced using an isometamidium-PTG conjugate as an immunogen, exhibited high levels of cross-reactivity.
    AGROVOC Keywords
    monoclonal antibodies; trypanosomiasis; drug therapy; isomerization; disease control; animal diseases
    Subjects
    ANIMAL DISEASES; DISEASE CONTROL; LIVESTOCK; VACCINES;
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