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dc.contributor.authorDulo, F.
dc.date.accessioned2014-09-25T15:51:00Z
dc.date.available2014-09-25T15:51:00Z
dc.date.issued2014-06-15
dc.identifier.citationDulo, F. 2014. Prevalence and antimicrobial resistance profile of Escherichia coli O157:H7 in goat slaughtered in Dire Dawa Municipal Abattoir as well as food safety knowledge, attitude and hygiene practice assessment among slaughter staff, Ethiopia. MSc thesis, Addis Ababa, Ethiopia: Addis Ababa University.en_US
dc.identifier.urihttp://hdl.handle.net/10568/43770
dc.description.abstractEscherichia coli O157:H7 serotype is worldwide zoonotic pathogens responsible for the majority of severe cases of human enterohemorrhagic Escherichia coli (EHEC) disease. The aim of this study was to investigate the prevalence and antimicrobial resistance pattern of E. coli O157:H7 in goat slaughtered at Dire Dawa municipal abattoir, Ethiopia. A total of 235 samples were collected from cecal contents, carcass and environment sample (slaughter house worker’s hand, knife and carcass wash water) as 93, 93 and 49, respectively through the months of January and April. E. coli O157:H7 was identified by the method slightly modified to ISO 16654:2001.The samples were initially enriched in modified trypticase broth containing novobiocin supplement, followed by plating onto sorbitol MacConkey agar. Consequently, the suspected non-sorbitol fermenting (NSF) colonies were confirmed as E. coli biochemically using indole test and selected for serotyping. Out of 235 samples collected, the overall prevalence of 2.55% (comprising of 2.15%, 3.22% and 2.04 of cecal contents, carcass swab and environmental samples respectively) had positive results for Dryspot E. coli O157 latex test kit (Oxoid, DR120M). Eighteen different antibiogram belonging to 10 pharmacological groups including ampicillin, amoxycillin-clavulanic acid, cefotaxime, ceftriaxone, cefoxitin,cefuroxime Sodium, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, kanamycin, nalidixic acid, nitrofurantoin, norafloxacin, streptomycin, sulfamethoxazole-trimethoprim, sulfonamides cpds, tetracycline were used for antimicrobial susceptibility test. Resistance to erythromycin was noted in all of the isolates (100%, n=6/6). Most of E. coli O157:H7 isolates also showed high resistance to ampicillin (83.3%, n=5/6) and moderate resistance to nitrofurantoin (50%, n=3/6). All the isolates were resistant to at least two of the antibiotics tested. No isolated E. coli O157:H7 strain resistance noted to cefotaxime, ceftriaxone, cefuroxime sodium, chloramphenicol, ciprofloxacin, gentamicin nalidixic acid and norfloxacin. This study concludes that the occurrence of E. coli O157:H7 and E. coli O157:H7 multiple antibiotic resistant profiles in goat slaughtered at Dire Dawa municipal abattoir and this may show a risk for public health and food safety. Regulatory control of antibiotics usage in livestock production, meat hygiene and pharmaco-epidemiological surveillance in food animals is hereby recommended to ensure consumer safety.en_US
dc.language.isoenen_US
dc.publisherAddis Ababa Universityen_US
dc.subjectGOATSen_US
dc.subjectFOOD SAFETYen_US
dc.subjectETHIOPIAen_US
dc.titlePrevalence and antimicrobial resistance profile of Escherichia coli O157:H7 in goat slaughtered in Dire Dawa Municipal Abattoir as well as food safety knowledge, attitude and hygiene practice assessment among slaughter staff, Ethiopiaen_US
dc.typeThesisen_US
cg.subject.ilriFOOD SAFETYen_US
cg.subject.ilriGOATSen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationAddis Ababa University
cg.targetaudienceSCIENTISTSen_US
cg.fulltextstatusGrey Literatureen_US
cg.placeAddis Ababa, Ethiopiaen_US
cg.coverage.regionAFRICA
cg.coverage.regionEAST AFRICA
cg.coverage.countryETHIOPIA
cg.contributor.crpAGRICULTURE FOR NUTRITION AND HEALTHen_US


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