Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken
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Mobegi, A.V. 2006. Mitochondrial DNA D-loop sequences reveal the genetic diversity of African chicken. In: Rege, J.E.O.; Nyamu, A.M.; Sendalo, D. (eds.). 2006. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Proceedings of the 4th All Africa Conference on Animal Agriculture and the 31st annual meeting of Tanzania Society for Animal Production, Arusha, Tanzania, 20-24 September 2005. Dar es Salaam, Tanzania: TSAP and Nairobi, Kenya: ILRI.
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Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of African domestic chicken. A total of 398 individuals belonging to 28 populations were sampled from 12 African countries. The hypervariable 1 (HV1) segment of the D-loop was PCR amplified and subsequently sequenced. The sequences of the first 397 nucleotides were used for analysis. Fifty-two haplotypes were identified from 50 polymorphic sites with polymorphism between nucleotides 167 and 397 contributing to 96% of the variation. Phylogenetic analysis indicates that African domestic chicken mtDNA can be grouped into six distinct clades with one to four clades observed in populations. AMOVA analysis indicates that 64.8% of the total sequence variation between haplotypes was present within population and 35.2% between populations. Our results suggest multiple maternal origins for the African domestic chicken.