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    A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs

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    Authors
    Svitek, Nicholas
    Nzau, Benjamin M.
    Steinaa, Lucilla
    Nene, Vishvanath
    Date
    2015-04
    Language
    en
    Type
    Journal Article
    Review status
    Peer Review
    ISI journal
    Accessibility
    Limited Access
    Metadata
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    Citation
    Svitek, N., Nzau, B., Steinaa, L. and Nene, V. 2015. A method to discriminate between closely related bovine major histocompatibility complex class I alleles by combining established PCR-SSP assays with RFLPs. Tissue Antigens 85(4):278-282.
    Permanent link to cite or share this item: https://hdl.handle.net/10568/66014
    DOI: https://doi.org/10.1111/tan.12524
    Abstract/Description
    We have developed a polymerase chain reaction-sequence-specific primers-restriction fragment length polymorphism (PCR-SSP-RFLP) method to rapidly differentiate between the A18 and A18 variant (v) BoLA haplotypes and between A14 and A15/A15v BoLA haplotypes in Holstein/Friesian cattle. We used published SSP to PCR amplify BoLA alleles expressed in animals of known haplotype and exposed the amplicons to the restriction enzyme PvuII that was predicted to cut at a unique site in the middle of BoLA-6*01302 (A18v) and BoLA-1*00901(A15) but not in BoLA-6*01301 (A18) or BoLA-1*02301 (A14) alleles. Whereas the method does not discriminate between the A15 and A15v haplotypes, as the BoLA-1*00902 allele associated with A15v also contains a PvuII site, we are interested in cattle of A18 and A14 haplotype for vaccine related studies. Our results also indicated that the BoLA-6*01302 (A18v) allele is much more abundant than BoLA-6*01301 (A18) in the cattle that we sampled.
    CGIAR Author ORCID iDs
    Lucilla Steinaahttps://orcid.org/0000-0003-3691-3971
    Other CGIAR Affiliations
    Livestock and Fish
    AGROVOC Keywords
    theileria; cattle; animal diseases; vaccines; disease control; east coast fever
    Subjects
    ANIMAL DISEASES; CATTLE; DISEASE CONTROL; ECF; LIVESTOCK; VACCINES;
    Species
    Theileria parva
    Organizations Affiliated to the Authors
    International Livestock Research Institute
    Collections
    • ILRI articles in journals [5969]
    • ILRI vaccine biosciences program outputs [99]
    • Improved vaccines for the control of East Coast fever in cattle in Africa [49]
    • Livestock Fish Flagship: Animal Health [71]
    • Livestock Fish journal articles [290]

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