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dc.contributor.authorObara, I.en_US
dc.contributor.authorUlrike, S.en_US
dc.contributor.authorMusoke, A.J.en_US
dc.contributor.authorSpooner, P.R.en_US
dc.contributor.authorJabbar, A.en_US
dc.contributor.authorOdongo, D.en_US
dc.contributor.authorKemp, Stephen J.en_US
dc.contributor.authorSilva, J.C.en_US
dc.contributor.authorBishop, Richard P.en_US
dc.date.accessioned2015-05-12T09:27:52Zen_US
dc.date.available2015-05-12T09:27:52Zen_US
dc.date.issued2015-05-01en_US
dc.identifier.citationObara, I., Ulrike, S., Musoke, T., Spooner, P. R., Jabbar, A., Odongo, D., Kemp, S., Silva, J. C. and Bishop, R. P. 2015. Molecular evolution of a central region containing B cell epitopes in the gene encoding the p67 sporozoite antigen within a field population of Theileria parva. Parasitology Research 114(5):1729-1737.en_US
dc.identifier.issn1432-1955en_US
dc.identifier.urihttps://hdl.handle.net/10568/66016en_US
dc.description.abstractProtective immunity induced by the infective sporozoite stage of Theileria parva indicates a potential role for antibodies directed against conserved serologically reactive regions of the major sporozoite surface antigen p67 in vaccination to control the parasite. We have examined the allelic variation and determined the extent of B cell epitope polymorphism of the gene encoding p67 among field isolates originating from cattle exposed to infected ticks in the Marula area of the rift valley in central Kenya where the African cape buffalo (Syncerus caffer) and cattle co-graze. In the first of two closely juxtaposed epitope sequences in the central region of the p67 protein, an in-frame deletion of a seven-amino acid segment results in a truncation that was observed in parasites derived from cattle that co-grazed with buffalo. In contrast, the variation in the second epitope was primarily due to nonsynonymous substitutions, resulting in relatively low overall amino acid conservation in this segment of the protein. We also observed polymorphism in the region of the protein adjacent to the two defined epitopes, but this was not sufficient to provide statistically significant evidence for positive selection. The data indicates that B cell epitopes previously identified within the p67 gene are polymorphic within the Marula field isolates. Given the complete sequence identity of the p67gene in all previously characterized T. parva isolates that are transmissible between cattle by ticks, the diversity observed in p67 from the Marula isolates in combination with the clinical reaction of the infected cattle is consistent with them originating from ticks that had acquired T. parva from buffalo.en_US
dc.language.isoenen_US
dc.sourceParasitology Researchen_US
dc.subjectANIMAL DISEASESen_US
dc.subjectDISEASE CONTROLen_US
dc.titleMolecular evolution of a central region containing B cell epitopes in the gene encoding the p67 sporozoite antigen within a field population of Theileria parvaen_US
dc.typeJournal Articleen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriGENETICSen_US
cg.subject.ilriVACCINESen_US
cg.identifier.statusLimited Accessen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationUniversity of Marylanden_US
cg.contributor.affiliationResearch Center Borstelen_US
cg.identifier.doihttps://dx.doi.org/10.1007/s00436-015-4358-6en_US
cg.isijournalISI Journalen_US
cg.contributor.crpLivestock and Fishen_US


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