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    Re-evaluation of Yam Mosaic Virus (YMV) detection methods

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    Authors
    Eni, A
    Hughes, J.
    Asiedu, R.
    Rey, M.
    Date
    2012
    Language
    en
    Type
    Journal Article
    Review status
    Peer Review
    Accessibility
    Limited Access
    Metadata
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    Citation
    Eni, A., Hughes, J., Asiedu, R., & Rey, M. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences, 5(1), 18-22.
    Permanent link to cite or share this item: http://hdl.handle.net/10568/79829
    DOI: https://dx.doi.org/10.5829/idosi.ajps.2012.5.1.10512
    Abstract/Description
    Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable.
    CGIAR Affiliations
    Roots, Tubers and Bananas
    AGROVOC Keywords
    YAM MOSAIC VIRUS; DETECTION SENSITIVITY; GENUS POTYVIRUS; YAM; DIOSCOREA; EPIDEMIOLOGICAL; ISOLATION; ALKALINE PHOSPHATASE
    Subjects
    YAM; PLANT DISEASES
    Countries
    BENIN; GHANA
    Regions
    AFRICA; WEST AFRICA
    Collections
    • IITA Journal Articles [1807]
    • RTB Journal Articles [656]

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