High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding
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Mengistu, D.; Kidane, Y.; Catellani, M.; Frascaroli, E.; Fadda, Pe, E.; C.; Dell'Acqua, M. (2016) High-density molecular characterization and association mapping in Ethiopian durum wheat landraces reveals high diversity and potential for wheat breeding. Plant Biotechnology Journal, 14, p.1800-1812 ISSN: 1467-7652
Permanent link to this item: http://hdl.handle.net/10568/79892
Internet URL: http://www.bioversityinternational.org/e-library/publications/detail/high-density-molecular-characterization-and-association-mapping-in-ethiopian-durum-wheat-landraces-reveals-high-diversity-and-potential-for-wheat-breeding/
Durum wheat (Triticum turgidum subsp. durum) is a key crop worldwide, and yet, its improvement and adaptation to emerging environmental threats is made difﬁcult by the limited amount of allelic variation included in its elite pool. New allelic diversity may provide novel loci to international crop breeding through quantitative trait loci (QTL) mapping in unexplored material. Here, we report the extensive molecular and phenotypic characterization of hundreds of Ethiopian durum wheat landraces and several Ethiopian improved lines. We test 81 587 markers scoring 30 155 single nucleotide polymorphisms and use them to survey the diversity, structure, and genome-speciﬁc variation in the panel. We show the uniqueness of Ethiopian germplasm using a siding collection of Mediterranean durum wheat accessions. We phenotype the Ethiopian panel for ten agronomic traits in two highly diversiﬁed Ethiopian environments for two consecutive years and use this information to conduct a genome-wide association study. We identify several loci underpinning agronomic traits of interest, both conﬁrming loci already reported and describing new promising genomic regions. These loci may be efﬁciently targeted with molecular markers already available to conduct marker-assisted selection in Ethiopian and international wheat. We show that Ethiopian durum wheat represents an important and mostly unexplored source of durum wheat diversity. The panel analysed in this study allows the accumulation of QTL mapping experiments, providing the initial step for a quantitative, methodical exploitation of untapped diversity in producing a better wheat.