Evidence for a latent viruslike agent in cassava
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GABRIEL, C.J.; WALSH, R.; NOLT, B.L. 1987. Evidence for a latent viruslike agent in cassava. Phytopathology. 77(1):92-95.
Permanent link to this item: http://hdl.handle.net/10568/95254
Internet URL: http://www.apsnet.org/phyto/PDFS/1987/Phyto77n01_92.PDF
Several species of double-stranded RNA (dsRNA) were found to occur in the cassava clone Secundina, which was thought to be virus- free. A large dsRNA molecule (6.6 x 106 Mr = relative molecular mass)(L-dsRNA) was shown to be graft, but not mechanically transmitted and occurred primarily in a nuclear-rich fraction isolated from dsRNA-containing Secundina plants. Viruslike particles were not detected in plants containing the L-dsRNA using a var. of purification procedures. L-dsRNA was also found in 2 non- Secundina cassava introductions. Smaller dsRNAs(S-dsRNAs), ranging mainly between 0.61-0.41 x 106 Mr, were also detected in some Secundina plants containing the L-dsRNA. The S-dsRNAs were not transmissible by mechanical inoculation or grafting. All dsRNAs could be eliminated by meristem-tip culture of infected plants. DsRNA analysis is recommended for indexing cassava for this latent viruslike agent. (AS)